Fall Semester Learning Objectives
for Principles & Research Methods
 (Last Modified on: August 15, 2006 )

Basic Principles & Inflammation Block ANS & CV Pharm Block
Drug Receptor Interactions (Principles) Renal Excretion (Principles)
Signal Transduction (Principles) ECG (Res Methods)
Cytochrome P-450 (Principles) Methods in CV Research (Res Methods)
Nitric Oxide & Arachidonic Acid (Principles) Methods in Smooth Muscle Pharm (Res Methods)
Reactive Oxygen Species (Principles)  
   
Neoplasia & Cancer Chemotherapy Block Pulmonary & Molecular Biology (Part 1) Block
Mechanisms of Chemical Carcinogenesis (Principles) Northern, Western & Southern Blots (Pharm Res)
Methods in Chemical Carcinogenesis (Pharm Res) RIA & Elisa (Pharm Res)
Angiogenesis (Principles) Methods in Pulmonary Pharm (Pharm Res)
Tumor Promotion & Prevention (Principles) DNA Recombinant Technology (Pharm Res)
Anticancer Drug Development (Pharm Res) Mechanisms of the Immune Response (Principles)
  Tissue Culture Techniques (Pharm Res)
   
Antimicrobial Block (& Foundation for ANS / CV) GI & Molecular Biology (Part 2) Block
Microbial & Eucaryotic Drug Resistance (Principles) Gene Transfer Technology (Pharm Res)
Ion Channels (Principles) Synthesis of Nucleic Acids (Principles)
Excitable Membranes (Principles) Electrophoresis & Immunoblotting (Pharm Res)
Smooth Muscle (Principles) Inhibition of Gene Expression (Principles)
Skeletal Muscle (Principles) DNA Sequencing (Pharm Res)
Cardiac Muscle (Principles) Monoclonol Antibodies (Pharm Res)
  Methods in Stem Cell Biology (Pharm Res)
  Gene Array & Proteomics (Pharm Res)
  Chemical Analysis - HPLC, Mass Spec (Pharm Res)
  Immunohistochemistry (Pharm Res)
   

Basic Principles & Inflammation Block

Drug Receptor Interactions (Principles) Dr. Kadowitz

By the end of this session, you should be able to:

  1. List several different types of drug receptors.
  2. Define “affinity” and “efficacy”. Compare & contrast these terms.
  3. Explain why the dose response relationship for receptor occupancy may have a lower EC50 value compared to the
  4. EC50 for drug response in response to the concept of spare receptors.
  5. Define “potency”.
  6. Describe the major characteristics of an agonist & an antagonist.
  7. Explain the difference between a full agonist & a partial agonist.
  8. Describe the effects of a competitive & a non-competitive antagonist on the dose response curve of an agonist.
  9. Define “desensitization” and explain the difference between homologous and heterologous desensitization.
  10. Describe how ED50, TD50 and LD50 are measured, as well as their meaning.
  11. Explain the meaning of “Therapeutic Index” an define how it is determined.
  12. Describe the basic structure of a G-protein coupled receptor.
  13. Describe the steps involved in G-protein mediated signal transduction.
  14. List the basic features of a tyrosine kinase receptor & how signal transduction is mediated by this receptor subtype.
  15. Describe how drug binding to a receptor associated with an ion channel can initiate a cellular response.
  16. List two types of intracellular receptors.
  17. Explain how “signal amplification” can occur.
Signal Transduction Dr. Beckman

By the end of this session, you should be able to:

  1. Describe some complementary sets of receptor proteins in each cell that enable it to bind and respond to signal molecules in a characteristic way.
  2. Explain how a molecular switch works.
  3. List eight types of signaling proteins.
  4. Describe five ways in which target cells can become desensitized to a signal molecule.

 

Cytochrome P-450 Dr. Agrawal

By the end of this session, you should be able to:

  1. Describe the distribution of P-450 enzymes.
  2. Describe the isolation of microsomal and mitochondrial fractions.
  3. Explain the electron transport system in the microsomal drug metabolizing system.
  4. Discuss the differences between P-450 mediated oxidative and reducing reactions.
  5. List various types of major human P-450 isozymes and specific substrates.
  6. Explain P-450 enzyme induction and inhibition by drugs and their interaction.
Endothelial Metabolism - Nitric Oxide & Arachidonic Acid (Principles) Dr. Kadowitz

By the end of this session, you should be able to:

Nitric Oxide:

  1. Explain the chemical properties of NO.
  2. Describe the role of NO in the regulation of smooth muscle, nervous system & inflammatory cell function.
  3. Explain the signal transduction mechanisms by which NO regulates cellular function.
  4. Explain the role of alterations in NO formation in disease states.
Arachidonic Acid:
  1. Explain the role of omega-3 and omega-6 series polyunsaturated fatty acids in the formation of lipid mediators.
  2. Describe the role of prostaglandins, cyclooxygenase (Cox-1, Cox-2) and lipoxygenase products in physiological regulation of organ function.
  3. Explain the role of prostaglandin & lipoxygenase products in pathophysiologic states (arthritis, asthma).
  4. Describe the mechanism by which Cox and lipoxygenase inhibitors and antagonists are useful in the treatment of arthritis & asthma.
Reactive Oxygen Species (Principles) Dr. Mondal

By the end of this session, you should be able to:

  1. Describe the central role of ROS in normal metabolic functions, and in disease pathogenesis, e.g. inflammation, atherosclerosis, cancer, AIDS, aging, etc...
  2. List the major classes of ROS, their sources, and properties.
  3. Explain the role of Mitochondrial electron transport chain (ETC) in ROS generation.
  4. Describe the Fenton and Haber-Weiss reactions which generate hydroxyl radicals.
  5. Explain the deleterious consequences of ROS on DNA, proteins, and lipids.
  6. Describe the major anti-oxidant defence systems and their mechanisms of action.

 

 

 

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