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Experimental Physiology

GPSO 606

Course Director: Dr. Majid

Instructors:  Drs. Majid , Swanson, Johnson, Elmslie, Awada

Goals and Objectives:   The course will focus on the basic functions of cardiovascular, respiratory, renal, skeletal muscle and cellular transport systems using anesthetized animal models (pigs, rats, frogs etc) as well as human volunteers. The student will practice some basic simple surgical procedures in anesthetized animals and will formulate experimental design and research ideas aimed at strengthening the foundations of their medical, surgical and research training.

Students in this course will:
1) learn fundamental regulatory mechanisms of some basic organs in a living system.
2) practice simple surgical procedures on a living animal.
3) acquire basic experience in conducting biological research in-vivo using animal models.
4) consider the ethics of using animals in research.

Methods of Instruction: The course will consist of laboratory exercises, research orientation and group discussions. Students will work in a group under the guidance of the instructor.

Requirements: Medical/ graduate students who have taken/will take the Human Physiology course.

Length of course: 16 weeks

Class hours per week: Four hours (Friday, 1pm to 5pm)

Grading: Medical students will receive a grade of Satisfactory or Unsatisfactory to be determined on the basis of attendance and participation. Graduate students will receive a letter grade. Attendance is mandatory. Excused absences may be granted only by the Dean, Department Chair or instructors.

List of Topics

Session

Topic

Instructor

1

Introductory Session:
-Physiological measuring techniques; Assessment of Pressure-volume and pressure-flow relationship;
- General principles of the use of animals in experiments in physiology: (Introduction to surgical tools, principles of surgery, rules and discipline in animal experimentation)

Drs. Swanson/Majid

2
Experiment in Frog: Mechanisms of Na+ reabsorption by tight-epithelia. Dr. Awayda
3
- Experiment in Frog: Neuro-muscular function. Dr. Elmslie
4
- Experiment in rats: Langendorf Heart Preparation

Drs. Robert Johnson/
Fruzsina Johnson

5
Experiment in pig: Cardiovascular regulation. Drs. Majid/Swanson
6
Discussion on the experimental findings in cardiovascular regulation Dr. Majid
7
Quantitative cardiovascular physiology (QCP)
(Computer simulation of the circulatory system)
Design and conduct experiments on QCP
Drs. Swanson /Majid
8
Experiment in pig: Control of ventilation. Drs. Swanson/Majid
9
Discussion on the experimental findings in control of ventilation. Dr. Swanson
10
Experiment in pig: Renal excretory function. Drs. Majid/Swanson
11
Discussion on the experimental findings in renal excretory function. Dr. Majid
12
Regulation of water, electrolytes and pH by the kidneys in human Drs. Majid/Swanson
13
Experiments on Rabbit Smooth muscle Prep: GI experiments Dr. Solange Abdournur
14
Reproductive Physiology: Lab experiment Dr. Fruzsina Johnson
15
Reproductive Physiology – Field trip to Primate Research Center Dr. Harrison
16
Final evaluation/wrap-up session All Instructors

 

 
Department of Physiology
1430 Tulane Ave., New Orleans, LA 70112
504-988-5251; Fax # 504-988-2675