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MOLECULAR ANALYSIS OF MONOCLONAL ANTIBODIES
AND OTHER PROTEINS
Updated 3/27/05
1. R.C. Blake II and D.A. Blake (2005) “Quantitative analysis of antibody-antigen interactions using immobilized ligands; Kinetic exclusion assays are more accurate than surface plasmon resonance”, Progress in Monoclonal Antibody Research , (M.A. Simons, ed.) Nova Science Inc., Hauppauge , New York , in press.
2. R.C. Blake II, N. Ohmura, S.J. Lackie, X. Li, J.B. Delehanty, I.A. Darwish, and D.A Blake (2005) “Monoclonal antibodies that exhibit allosteric binding behavior”, Monoclonal Antibodies: New Research , (M.A. Simons, ed.) Nova Science Publishers, Inc., Hauppauge , New York , in press.
3. A.M. Kriegel and D.A. Blake (2005) “Antibody fragments with specificity for metal-chelate complexes from human semi-synthetic phage display libraries”, Recent Research Developments in Bioconjugate Chemistry , in press.
4. R.C. Blake II, A.R. Pavlov, M. Khosraviani, H.E. Ensley, G.E. Kiefer, H. Yu, X. Li, and D.A. Blake (2004) “Novel monoclonal antibodies with specificity for chelated uranium(VI): Isolation and binding properties”, Bioconjugate Chemistry 15 :1125-1136. PDF
5. R.C. Blake II and D.A. Blake (2003) “Kinetic exclusion assay to study high-affinity binding interactions in homogeneous solutions”, Methods in Molecular Biology , Vol. 248: Antibody Engineering: Methods and Protocols, (Benjamin K.C. Lo, Ed.) Humana Press, Totowa , New Jersey , pp 417-430.
6. J.B. Delehanty, R.M. Jones, T.C. Bishop and D.A. Blake (2003) "Identification of important residues in metal-chelate recognition by monoclonal antibodies", Biochemistry, 42:14173-14183. PDF
7. R.C. Blake II, J.B. Delehanty, M. Khosraviani, H. Yu, R.M. Jones, and D.A. Blake (2003) "Allosteric binding properties of a monoclonal antibody and its Fab fragment", Biochemistry, 42 :497-598. PDF
8. R.C. Blake II and D.A. Blake (2003) “Kinetic exclusion assay to study high-affinity binding interactions in homogeneous solutions”, Methods in Molecular Biology , Vol. 248: Antibody Engineering: Methods and Protocols, (Benjamin K.C. Lo, Ed.) Humana Press, Totowa , New Jersey , pp 417-430.
9. R.M. Jones, H. Yu, J.B. Delehanty, and D.A. Blake (2002) "Monoclonal antibodies that recognize minimal differences in the 3-dimensional structures of metal-chelate complexes", Bioconj. Chem., 13: 408-415. PDF
10. D.A. Blake, A.R. Pavlov, H. Yu, M. Khosraviani, H.E. Ensley, and R.C. Blake II (2001) "Antibodies and antibody-based assays for hexavalent uranium", Anal. Chim. Acta, 444:3-11.
11. M. Khosraviani, R.C. Blake II, A.R. Pavlov, S.C. Lorbach, H. Yu, J.B. Delehanty, M.W. Brechbiel, and D.A. Blake (2000) "Binding properties of a monoclonal antibody directed toward lead-chelate complexes", Bioconj. Chem., 11:267-277. PDF
12. R.C. Blake II, A.R. Pavlov, and D.A. Blake (1999) "Automated kinetic exclusion assays to quantify protein binding interactions in homogeneous solution", Anal. Biochem. 272:123-134. PDF
13. D.A. Blake, R.C. Blake II, M. Khosraviani, and A.R. Pavlov (1998) "Metal ion immunoassays", Anal. Chim. Acta, 376:13-19.
14. D.A. Blake, M. Khosraviani, A.R. Pavlov, and R.C. Blake, II (1997) "Characterization of a metal-specific monoclonal antibody", in Immunochemical Technology for Environmental Applications, Eds. Aga, D.S., and Thurman, E.M. American Chemical Society Symposium Series, Vol. 657, Washington D.C., pp. 49-60.
15. D.A. Blake, A.R. Pavlov, M. Khosraviani, and R.C. Blake, II (1997) Determination of antibody affinity and specificity, Proceedings of Immunochemistry Summit V and Joint National Conference for Environmental Health Monitoring, Ed. Joseph, J.M. Association of State and Territorial Public Health Laboratories, Washington, D.C., pp. 95-106.
16. A.R. Pavlov, M. Khosraviani, J. E. Chaiban, and D.A. Blake (1997) "Strategies and
problems in the isolation of metal-specific monoclonal antibodies",
Antibody Engineering II: New Technology, Application, and Commercialization,
Vol. II, IBC USA Publishing,
17. D.A. Blake, P. Chakrabarti, M. Khosraviani, F.M. Hatcher, C.M. Westhoff, P. Goebel, D.E. Wylie, and R.C. Blake, II (1996) "Metal binding properties of a monoclonal antibody directed towards metal-chelate complexes", J. Biol. Chem., 271:27677-27685.
18. R.C. Blake II, R.F. Vassall, and D.A. Blake (1989) "The Michaelis constants of a non-chromogenic substrate may be determined using a chromogenic substrate", Arch. Biochem. Biophys. 272:52-68.