Darwin Prockop, M.D., Ph.D. Professor of Biochemistry and Director, Center for Gene Therapy, Tulane University Health Sciences Center

University of Pennsylvania, M.D., 1956; George Washington University, Ph.D., Biochemistry, 1961.

Professor of Biochemistry, Director, Center for Gene Therapy, Tulane University Health Sciences Center. Molecular genetics of skeletal diseases, biology of stem cells, use of adult stem cells for the therapy of diseases such as osteoporosis, osteoarthritis, Parkinson's disease, and Alzheimer's disease. (E-MAIL add.: dprocko@tulane.edu)

• Pochampally RR, Neville BT, Schwarz EJ, Li MM, Prockop DJ. Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion. Proc Natl Acad Sci U S A. 2004 101:9282-5
• Prockop DJ. Targeting gene therapy for osteogenesis imperfecta. N Engl J Med. 2004 350:2302-4.
• Gregory CA, Gunn WG, Peister A, Prockop DJ. An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Anal Biochem. 2004 329:77-84.
• Spees JL, Gregory CA, Singh H, Tucker HA, Peister A, Lynch PJ, Hsu SC, Smith J, Prockop DJ. Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy. Mol Ther. 2004 9:747-56.
• Sekiya I, Larson BL, Vuoristo JT, Cui JG, Prockop DJ. Adipogenic differentiation of human adult stem cells from bone marrow stroma (MSCs). J Bone Miner Res. 2004 19:256-64.
• Peister A, Mellad JA, Wang M, Tucker HA, Prockop DJ. Stable transfection of MSCs by electroporation. Gene Ther. 2004 11:224-8.
• Pochampally RR, Smith JR, Ylostalo J, Prockop DJ. Serum deprivation of human marrow stromal cells (hMSCs) selects for a subpopulation of early progenitor cells with enhanced expression of OCT-4 and other embryonic genes. Blood. 2004 103:1647-52.
• Peister A, Mellad JA, Larson BL, Hall BM, Gibson LF, Prockop DJ. Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential. Blood. 2004 103:1662-8.
• Prockop DJ, Gregory CA, Spees JL. One strategy for cell and gene therapy: harnessing the power of adult stem cells to repair tissues. Proc Natl Acad Sci U S A. 2003 100 Suppl 1:11917-23.
• Gregory CA, Singh H, Perry AS, Prockop DJ. The Wnt signaling inhibitor dickkopf-1 is required for reentry into the cell cycle of human adult stem cells from bone marrow. J Biol Chem. 2003 278:28067-78.
• Prockop DJ. Further proof of the plasticity of adult stem cells and their role in tissue repair. J Cell Biol. 2003 160:807-9.
• Spees JL, Olson SD, Ylostalo J, Lynch PJ, Smith J, Perry A, Peister A, Wang MY, Prockop DJ. Differentiation, cell fusion, and nuclear fusion during ex vivo repair of epithelium by human adult stem cells from bone marrow stroma. Proc Natl Acad Sci U S A. 2003 100:2397-402.
• Hofstetter, C.P., Schwarz, E.J., Hess, D., Widenfalk, J., E.l., 2002. Manira A, Prockop DJ, Olson L. Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery. Proc Natl Acad Sci U S A. 99:2199-204.
• Prockop, D.J., 2002. Adult stem cells gradually come of age. Nat. Biotechnol. 20:791-2.
• Colter, D.C., Sekiya, I., Prockop. D.J., 2001. Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proceedings National Academy of Sciences. Proceedings of the National Academy of Sciences 98(14): 8741-7845.
• Javazon, E.H., Colter, D.C., Schwarz, E.J., Prockop, D.J., 2001. Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells. Stem Cells 19: 219-225, 2001.
• Colter D.C., Class R., DiGirolamo C.M., and Prockop, D.J. Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proceedings of the National Academy of Sciences USA 97:3213-3218, 2000.
• Schwarz E.J., Alexander, G.M., Prockop D.J., and Azizi, S.A. Multipotential marrow stromal cells transduced to produce L-DOPA: Engraftment in a rat model of Parkinson's disease. Human Gene Therapy 10:2539-2549, 1999.

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